Glycosylation in S2 cells is very much like that in SF9, Sf21, and High Five cells. The nature of Drosophila N-linked glycosylation is less complex than mammalian glycosylation – it is generally of the paucimannose type, and is not trimmed and sialylated. O-linked glycosylation is similar, although not identical, to mammalian cells. […]
Gentamycin 10 µg/mL Inhibits bacterial protein synthesis. Penicillin-Streptomycin 100 U/mL Penicillin Inhibits bacterial cell wall synthesis. 100 µg/mL Streptomycin Inhibits bacterial protein synthesis. Amphotericin B Fungizone 0.25 µg/mL Binds sterols and interferes with membrane permeability.
No, and in fact CO2 is toxic to S2 cells.
Humidification is only necessary for monolayer cultures in tissue culture plates. Plates can also be wrapped in parafilm and put in a box with a beaker with water. For other cultures, humidification is not necessary.
The accumulating dogma seems to indicate that CMV expresses in a number of cell types. For example, CMV drives expression in frog oocytes. The following articles discuss the CMV promoter in S2 cells. One provides evidence that it works well and one finds that it does not. Indicates that CMV […]
Yes. D. Mel is short for Drosophila melanogaster, which is the proper species name for the cells commonly referred to as Schneider S2 cells or just S2 cells.
We recommend 110 rpm for shake flasks with or without baffles.
S2 cells can be grown at 23-27 °C but they will recover after excursions up to 37 °C. However, we routinely grow our cells at 25 °C.
Yes, Baculovirus can infect S2 cells, however the virus will not replicate in the infected cells and will not be able to infect subsequent cells. No high-titer stocks can be generated from S2 cells.
Some of the advantages are: a more homogeneous glycosylation profile; better reproducibility between manufacturing runs; several options for cultivation modes, such as perfusion that allows for long cultivation times with daily harvests; no cell lysis, resulting in lower levels of contaminating host cell proteins.