Archives: FAQs

What is the S2 glycosylation like?

Glycosylation in S2 cells is very much like that in SF9, Sf21, and High Five cells. The nature of Drosophila N-linked glycosylation is less complex than mammalian glycosylation – it is generally of the paucimannose type, and is not trimmed and sialylated. O-linked glycosylation is similar, although not identical, to mammalian cells. […]

Does the CMV promoter drive expression in S2 cells?

The accumulating dogma seems to indicate that CMV expresses in a number of cell types. For example, CMV drives expression in frog oocytes. The following articles discuss the CMV promoter in S2 cells. One provides evidence that it works well and one finds that it does not. Indicates that CMV […]

Can baculovirus infect S2 cells?

Yes, Baculovirus can infect S2 cells, however the virus will not replicate in the infected cells and will not be able to infect subsequent cells. No high-titer stocks can be generated from S2 cells.

What are the advantages of ExpreS2 over the baculovirus system?

Some of the advantages are: a more homogeneous glycosylation profile; better reproducibility between manufacturing runs; several options for cultivation modes, such as perfusion that allows for long cultivation times with daily harvests; no cell lysis, resulting in lower levels of contaminating host cell proteins.