Glycosylation in S2 cells is very much like that in SF9, Sf21, and High Five cells. The nature of Drosophila N-linked glycosylation […]
Gentamycin 10 µg/mL Inhibits bacterial protein synthesis. Penicillin-Streptomycin 100 U/mL Penicillin Inhibits bacterial cell wall synthesis. 100 µg/mL Streptomycin Inhibits […]
No, and in fact CO2 is toxic to S2 cells.
Humidification is only necessary for monolayer cultures in tissue culture plates. Plates can also be wrapped in parafilm and put […]
The accumulating dogma seems to indicate that CMV expresses in a number of cell types. For example, CMV drives expression […]
Yes. D. Mel is short for Drosophila melanogaster, which is the proper species name for the cells commonly referred to as Schneider S2 cells […]
We recommend 110 rpm for shake flasks with or without baffles.
S2 cells can be grown at 23-27 °C but they will recover after excursions up to 37 °C. However, we […]
Yes, Baculovirus can infect S2 cells, however the virus will not replicate in the infected cells and will not be […]
Some of the advantages are: a more homogeneous glycosylation profile; better reproducibility between manufacturing runs; several options for cultivation modes, […]