What is the S2 glycosylation like?
Glycosylation in S2 cells is very much like that in SF9, Sf21, and High Five cells. The nature of Drosophila N-linked glycosylation […]
What antibiotics may be used in cell culture of S2 cells? What concentration should be used?
Gentamycin 10 µg/mL Inhibits bacterial protein synthesis. Penicillin-Streptomycin 100 U/mL Penicillin Inhibits bacterial cell wall synthesis. 100 µg/mL Streptomycin Inhibits […]
Does the incubator need to have CO2?
No, and in fact CO2 is toxic to S2 cells.
Does the incubator need to be humidified when culturing S2 cells?
Humidification is only necessary for monolayer cultures in tissue culture plates. Plates can also be wrapped in parafilm and put […]
Does the CMV promoter drive expression in S2 cells?
The accumulating dogma seems to indicate that CMV expresses in a number of cell types. For example, CMV drives expression […]
Are D. Mel-2 cells the same as Schneider S2 cells?
Yes. D. Mel is short for Drosophila melanogaster, which is the proper species name for the cells commonly referred to as Schneider S2 cells […]
What is the suggested speed for shake flasks for S2 suspension culture?
We recommend 110 rpm for shake flasks with or without baffles.
At what temperature can I grow S2 cells?
S2 cells can be grown at 23-27 °C but they will recover after excursions up to 37 °C. However, we […]
Can baculovirus infect S2 cells?
Yes, Baculovirus can infect S2 cells, however the virus will not replicate in the infected cells and will not be […]
What are the advantages of ExpreS2 over the baculovirus system?
Some of the advantages are: a more homogeneous glycosylation profile; better reproducibility between manufacturing runs; several options for cultivation modes, […]