s2 cellsIn 1972, I. Schneider succeeded in establishing embryonic Drosophila cell lines, which have been used for recombinant protein expression. Use of Drosophila cell lines proved to be a relevant addition to the protein expression toolbox available to scientists. Features of S2 cells include:

  • Derived from the late stages of Drosophila melanogaster (fruit fly) embryos (Schneider, 1972)
  • Doubling times of 18-24 hrs (both serum-containing and serum-free medium)
  • Cell size is 8-10 µm in diameter
  • Grow well between 5 and 350E6 cells/ml in suspension
  • Grow at 23 ºC to 27 ºC
  • Very robust, compatible with standard lab equipment