About S2 cells

s2 cellsIn 1972, I. Schneider succeeded in establishing embryonic Drosophila cell lines, which have been used for recombinant protein expression. Use of Drosophila cell lines proved to be a relevant addition to the protein expression toolbox available to scientists. Features of S2 cells include:

  • Derived from the late stages of Drosophila melanogaster (fruit fly) embryos (Schneider, 1972)
  • Doubling times of 18-24 hrs (both serum-containing and serum-free medium)
  • Cell size is 8-10 µm in diameter
  • Grow well between 5 and 350E6 cells/ml in suspension
  • Grow at 23 ºC to 27 ºC
  • Very robust, compatible with standard lab equipment