We offer fast assessment of expression feasibility in the ExpreS2 system, by transient screening expression of your proteins or protein variants: from transfection-ready DNA to a western blot result in 7 days. The feasibility studies are structured with go/stop decision points at critical steps. With ExpreS2, it takes only one week from DNA to protein detection.
The flexibility of performing transient expression, at a range of volumes from 96-deep well plates to liter scale, can fast track protein analytics. This provides fast access to protein, while the stable expressing pools, ready to use in 3-6 weeks, are the most efficient way of producing larger protein batches.
Transient screening: Find out in 7 days which of your proteins or protein variants are expressed. A range of options is then open for production of the selected proteins.
Stable expression screening: Starting with your gene(s) cloned into the ExpreS2 vectors, we will test in a transient expression assay how your protein(s) express, while initiating the stable expression experiment. Within 7 days, a go/stop decision is taken, based on the results from transient expression. Upon a “go” decision, results from stable expression will be available within 2-5 weeks. A go/stop decision at this point will be taken, regarding expansion of protein producing cell pool, possible selection of high expressers in ‘mini pools’ and the needed production volume.
Get in touch to discuss the needs of your feasibility study.