We have expressed a broad range of complex human, parasite and viral proteins in ExpreS2. Even proteins that have failed in other expression systems. We can also help you with a broad range of expression challenges, including heterodimers or trimers, virus-like particles (VLP) and low-expressing proteins, issues with broad glycosylation heterogeneity or issues with degradation of the desired protein by cellular proteases.
Stable protein expression
We can provide cell pellets/supernatants or purified protein from liter-scale cultures (shake flasks or bioreactors) in as little as 6 weeks after we receive your transfection-ready DNA. This service includes designing new gene constructs in our optimised expression vectors, stable cell line generation and protein production, which is verified using western blot analysis. We can supply you with the unpurified protein(s) or further purify them.
Services we provide
- Transient expression
- Medium throughput screening service
- Establishment of stable expression pools
- Production re-batching
- Selection of high-producing mini-pools and monoclonal cell lines
- Protein Purification
ExpreS2 TR, ExpreS2ion’s transfection reagent has been formulated and optimised for insect cell transfection. The performance of ExpreS2 TR in Sf9, Sf21, and S2 cells is superior to other commonly used transfection reagents, when compared head-to-head. ExpreS2 TR has also shown to be superior in transfection of mosquito cell lines.
Key applications and features
- Works perfectly in serum-containing and serum-free conditions
- Mix and add to cells – no need to change medium
- Customised batch sizes are available
To discuss your needs and hear more about the possibilities, get in touch.